WGA2

Sigma

 

GenomePlex® Complete Whole Genome Amplification (WGA) Kit

Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
Other NotesThe sequences of the universal primers provided in this kit are considered proprietary.
Features and Benefits• Successfully produces a negative control sample without product
• Flexibility to study DNA from any source
• No detectable locus or allele bias
• Compatible with a variety of microarray, capillary, and homogenous platforms for sequencing, genotyping, CGH, FISH, ChIP
• Increased sensitivity and accuracy for population studies, mutation discovery, and pharmacogenomics
General descriptionWGA is suitable for use with genomic DNA, blood, soil, plant, buccal swabs, cultured cells, and FFPE tissues. Amplification requires only a small amount of starting material (1 μl blood, single cheek swab, 10 ng of gDNA, or minimum 10 ng of damaged DNA), which after PCR produces a yield of 5-10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABIs TaqMan® assays, microsatellite analysis, or sequencing.
Legal InformationUse of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as the patented 5′ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
 TaqMan is a registered trademark of Roche Molecular Systems, Inc.
 GenomePlex is a registered trademark of Rubicon Genomics, Inc.

Properties

shipped inwet ice
storage temp.−20°C

Safety

Hazard CodesB

Related Products

Related productZ719218, Whole Genome Amplification: Methods Express Series
Comparable productWGA1, GenomePlex® Whole Genome Amplification (WGA) Kit
 WGA3, GenomePlex® WGA Reamplification Kit

References

referenceBarker, D. L., et al., Two methods for whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel Genome Res. 14, 901-907, (2004) Abstract
 Gribble, S., et al., Chromosome paints from single copies of chromosomes Chromosome Res. 12, 143-151, (2004) Abstract
 Thorstenson, Y. R. et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing Genome Res. 8, 848-855, (1998) Abstract
 Park, J. W., et al., Comparing Whole-Genome Amplification Methods and Sources for Biological Samples for Single-Nucleotide Polymorphism Genotyping Clin. Chem. 51(8), 1520-1523, (2004)